Signal transduction--single cell techniques

Cover of: Signal transduction--single cell techniques |

Published by Springer in Berlin, New York .

Written in English

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  • Cellular signal transduction -- Laboratory manuals.,
  • Cytology -- Laboratory manuals.

Edition Notes

Includes bibliographical references and index.

Book details

StatementBert Van Duijn, Anneke Wiltink, (eds.).
SeriesSpringer lab manual
ContributionsDuijn, Bert van, 1961-, Wiltink, Anneke, 1961-
LC ClassificationsQP517.C45 S556 1998
The Physical Object
Paginationxxvii, 468 p. :
Number of Pages468
ID Numbers
Open LibraryOL675147M
ISBN 103540625631
LC Control Number97021326

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Signal Transduction - Single Cell Techniques (Springer Lab Manuals): Medicine & Health Science Books @ A variety of powerful techniques to monitor and analyse the events during signal transduction at the single cell level are described in this lab manual.

An introductionary section for cell handling includes guidelines for constructing a perfusion chamber. A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber.

A main section of the book presents. The book is essential reading for students learning about signal transduction for the first time.

It will also be a vital reference for all cell, molecular, and developmental biologists and pharmacologists, neurobiologists, and immunologists studying processes regulated by cell signaling.

Studying signal transduction at the single-cell level. Advancements in observing single-cells, computational modeling, and techniques to manipulate cells in a spatiotemporal manner enable insights into signal transduction at the single-cell by:   Medical books Signal Transduction - Single Cell Techniques.

An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. Download E-books Signal Transduction — Single Cell Techniques PDF Nonfiction Comments: 0 By Can Ince (auth.), Bert Van Duijn, Anneke Wiltink (eds.).

Buy Signal Transduction - Single Cell Techniques (Springer Lab Manuals) by Bert Van Duijn (ISBN: ) from Amazon's Book Store. Everyday low prices and free delivery on Format: Paperback. Overview of Cell Signaling. Cell signaling can be divided into 3 stages. Reception: A cell detects a signaling molecule from the outside of the cell.

A signal is detected when the chemical signal (also known as a ligand) binds to a receptor protein on the surface of the cell or inside the cell. Perception of a Signal and the Transmission of the Signal into the Cell. Another principle of cell signalling is the ability of cells to perceive and correctly respond to signals in their environment.

To do this, they must first have specific receptors located on either the cell surface or within the cell. 18 Cell Signaling and Stress Responses, Go¨khan S. Hotamisligil and Roger J. Davis 19 Cell Death Signaling, Douglas R. Green and Fabien Llambi 20 Subversion of Cell Signaling by Pathogens, Neal M.

Alto and Kim Orth 21 Signal Transduction in Cancer, Richard Sever and Joan S. Brugge 22 Outlook, Jeremy Thorner, Tony Hunter.

Signal Transduction — Single Cell Techniques; Signal Transduction: Pathways, Mechanisms and Diseases; Signal Transforms in Dynamic Measurements; Signal and Image Analysis for Biomedical and Life Sciences; Signal and Image Processing Techniques for the Development of Intelligent Healthcare Systems; Signal and Image Processing for Biometrics.

In its broadest context, cell signaling involves the transduction of some event into another event. In sensory transduction, a sensory cell is exposed to some external signal that is transduced to produce a nervous signal, the action potential.

As we will see later in Chapterthis action potential can move along cell membranes to rapidly convey the signal, the action potential, to remote. Get this from a library. Signal transduction--single cell techniques. [Bert van Duijn; Anneke Wiltink;] -- A variety of powerful techniques to monitor and analyse the events during signal transduction at the single cell level are described in this lab manual.

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Book reviews Signal transduction ^ single cell techniques; Edited by B. Van Duijn and A. Wiltink, Springer-Verlag; Berlin, Heidelberg, New York, xx+ pp. DM (pb). ISBN This volume from the Springer Lab Manual series is comprised of three parts covering ‘Handling of cells in single cell experiments‘, ’Ion.

Signal Transduction • The cell senses extra cellular signals: – Hormones, pheromones, heat, cold, light, osmotic pressure, concentration change of glucose, K+, Ca2+ or cAMP.

• and commutes them in intracellular signals: – Signalling involves the same type of. [1] Van Dujin, Wiltink, “ Signal Transduction, Single Cell Techniques”, ISBNSpringer Verlag [2] Neher, “Elektronische Messtechnik in der Physiologie”. –Cell surface receptors receive the signal (e.g.

a messenger sub-stance) at the outside of the cell, become activated and initiate a signaling chain in the interior of the cell. In such signaling path-ways, the membrane-bound receptor transduces the signal at the cell membrane so that it is not necessary for the signal to actually enter the cell.

Search the world's most comprehensive index of full-text books. My library. Author(s): Duijn,Bert van,; Wiltink,Anneke, Title(s): Signal transduction - single cell techniques/ Bert Van Duijn, Anneke Wiltink, (eds.). It includes coverage of all important aspects of cell signaling, ranging from prokaryotic signal transduction to neuronal signaling.

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Springer Lab Manuals: Signal Transduction -- Single Cell Techniques (Paperback) Image analysis and flow cytometry are complementary techniques which provide new insights into essential aspects of cell biology.

This book presents an up-to-date overview of current ideas and methods in these domains. It consists of three parts. Part I 9 pins. We have leveraged single-cell RNA sequencing techniques to identify conserved patterns of cell-cell cross-talk in adult mammalian lungs, analyzing mouse, rat, pig, and human pulmonary tissues.

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Signal transduction--single cell techniques [ The book also contains a critical comparison of other extraction methods and discusses different coupling techniques.

The book helps the novice to adopt this method in his laboratory and provides the experienced practitioner with ideas and information necessary to optimise this successful. MCQ on Signal Transduction (Molecular Biology MCQ – 02) Dear Students, Welcome to Molecular Biology MCQ (Signal Transduction).This MCQ set consists of Molecular Biology Multiple Choice Questions from the topic Cell Signal Transduction and Cell-Cell Communication Mechanisms with Answer Key.

These questions can be used for the preparation of all the competitive examinations in. Cell-type-specific PTM analysis of colorectal cancer organoid cocultures reveals that shApc, Kras G12D and Trp53 RH cell-autonomously mimic signaling states normally induced by stromal fibroblasts and macrophages.

These results demonstrate how standard mass cytometry workflows can be modified to perform high-throughput multivariate cell-type. A signal transduction in biology is a cellular mechanism.

It converts a stimulus into a response in the cell. There are two stages in this process: A signalling molecule attaches to a receptor protein on the cell membrane.; A second messenger transmits the signal into the cell, and a change takes place in the cell.

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11 Feasibility structure–fluidic level determination by imped-ance measurement (H. To validate the SCIPhos assay, signal transduction in a cytokine-dependent cell line was examined simultaneously by both SCIPhos assays and conventional Western blot analysis.

After cytokine deprivation, TPO-dependent 32D/Mpl cells were stimulated with TPO, and phosphorylation of STAT5 and Jak2 was quantitatively measured. Cell-surface receptors receive the signal (e.g., a messenger substance) at the outside of the cell, become activated, and initiate signaling events in the interior of the cell.

In such signaling pathways, the membrane-bound receptor transduces the signal at the cell membrane so that it is not necessary for the signal to actually enter the cell.

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